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Chronic Diseases Prevention Review

Cancer Cell Research













Chronic  Diseases Prevention Review



Cancer Cell Research

Article: Polyene Phosphatidylcholine reverses Oxaliplatin resistance in human gastric cancer BGC823/L-OHP cell lines

by Ronghui Yuan

Cancer Cell Research 2018 5(19) 456-463; published online  5  April 2018

Abstract: This study investigated the effect of polyenoic phosphatidylcholine (PPC) on the proliferation and apoptosis of BGC823/L-OHP cells in an attempt to reverse the mechanism of drug resistance in these cells. Cell counting (CCK-8) was performed to test the ability of PPC and Oxaliplatin (L-OHP; 1, 5, 10, 20, 30, 40µg/ml) can inhibit the growth of gastric cancer cell lines BGC823 and BGC823/L-OHP. The IC50 was calculated. PPC promoted the growth of BGC823/L-OHP cells in the range of 0.5–24µM and inhibited cell growth in the range of 48–86µM. The L-OHP IC50 in BGC823 cells was 3.52µg/ml, while in BGC823/L-OHP cells was 28.53µg/ml. The results indicated that BGC823/L-OHP cells had a strong drug resistance (P<0.05). The L-OHP IC50 of BGC823/L-OHP cells treated with 5µmol/L PPC was 6.63µg/ml. Flow cytometry examination showed that PPC promoted apoptosis in BGC823/L-OHP cells. We also examined the expression levels of TLR4, Nanog, and ABCF2 by RT-PCR and Western blot, and determined that PPC can downregulate the expression of TLR4, Nanog and ABCF2 in resistant strains. PPC can increase the sensitivity of human gastric cancer resistant cells to oxaliplatin. Reversing the effect of oxaliplatin resistance, and this reversal occurs with a reduction in the levels of TLR4, Nanog, and ABCF2.

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Review: Indoor air pollution, lung cancer and solutions

by Hongwei Si

Cancer Cell Research 2018 5(19) 464-470; published online  5  April 2018

Abstract: About 90% of our time is spent indoors where we are exposed to chemical and biological contaminants and possibly to carcinogens. These agents are highly associated with the increased rates of nonspecific respiratory and neurologic symptoms, allergies, asthma and lung cancer. We reviewed the sources, health effects and control strategies for these agents, particularly the major carcinogens from contaminated indoor air. While the fundamental approaches including quitting smoking and using natural and healthy building materials are essential of eliminating indoor air contamination, some simple measures such as increasing ventilation in the central heating, ventilation and air-conditioning systemsand daily opening windows may be realistic, convenient and cost-effective ways to improve indoor air quality and health for homeowners.

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Chronic Diseases Prevention Review

Article: Isatin inhibits FAK signaling pathway in neuroblastoma

by Ya’nan Hua, Shengmin Zhao, Lin Hou

Chronic Diseases Prevention Review 2018 2(6) 6-9; published online 25 March 2018

Abstract: Isatin has been shown to initiate apoptotic processes in neuroblastoma SH-SY5Y cells. Immunohistochemical (IHC) results indicated that 73% samples of neuroblastoma have detected FAK, and the phosphorylation of p125FAK increased obviously in stage IV tumors. Therefore, this study aimed to investigate whether isatin inhibited FAK signaling pathway in SH-SY5Y cells. According to the results, isatin reduced the levels of phosphorylated FAK following treatment with isatin for 48 h. In addition, isatin significantly inhibited MMP9 expression. These effects may be exerted by isatin via down-regulating the expression level of p-FAK.

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Article: Protective effect of taurine against Aβ1-40-induced toxicity

by Yuxue Liu, Lei Wang, Chuanxia Ju, Fang Zhang, zhihong Yang, Fengxiao Li, Yan Guo, Ying Yan, Xu Yao

Chronic Diseases Prevention Review 2018 2(6) 10-15; published online 25 March 2018

Abstract:  In this study, we evaluated the effects of taurine against Aβ1-40 -induced neurotoxicity in vivo and in vitro experiments. Rats received an intracerebroventricular fusion of Aβ1-40 and were given taurine by intragastric administration. Then behavioral performance was assessed, which revealed that 3.2, 4.79mmol/kg taurine treatment promoted positive cognitive effects in Aβ1-40-induced rats. Further, an Aβ1-40-induced toxicity in human neuroblastoma SH-SY5Y cell model was used for investigating the mechanisms of taurine. Exposure of SH-SY5Y cells to Aβ1-40 caused cell apoptosis, reduction in cell viability and mitochondrial membrane potential (△Ψm). Pretreatment of the cells with 8, 20mmol/L taurine before Aβ1-40 exposure significantly attenuated those changes in a dose-dependent manner. These results show that taurine has significant protective effects against Aβ1-40 -induced toxicity in rats and SH-SY5Y cells, and the action mechanism may be associated with up-regulating mitochondrial membrane potential.

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