25. 10. 2019   

Chronic Diseases Prevention Review (Online ISSN: 2158-0820)


Current Issue

Vol.3  No.12


Article: Vivo study of MSCs on EPCs cells in ischemic heart disease
by  Jianming Wang, Gengbiao Chen, Youfeng Guo, Xiaohong Yin, Xiaodong Wang
Chronic Diseases Prevention Review 2019 3(12) 1-5; published online  18 July 2019
Abstract:  The treatment of ischemic heart disease mainly depends on the formation of blood vessels and the survival of cardiac myocytes. This paper will research the enhancement of EPC cells by mesenchymal stem cells in the treatment of ischemic heart disease. Mouse bone marrow was collected, MSCs cells were isolated and cultured. Flow cytometry was used to test the expression of CD34 and CD44. Then MSCs cells and endothelial progenitor cells co-culture 48 h, and 24 Wistar rats were randomly divided into EPCs group, EPCs MSCs + group and the control group. MSCs 2×106, EPCs 2×106, EPCs 2×106, and PBS buffer were injected into five different regions around ischemic myocardium. The changes in cardiac function were assessed by echocardiography after 4 weeks. MSCs 2×106, EPCs 2×106, EPCs 2×106, and PBS buffer were injected into five different regions around ischemic myocardium. The changes in cardiac function were assessed by echocardiography after 4 weeks. The rats were sacrificed, and the expression of VEGF was detected by immunohistochemistry and immunofluorescence. The surface markers CD34 and CD44 of MSCs and EPCs were differentially expressed. We successfully isolated MSCs and EPCs cells. Meanwhile, the proliferation index Ki67 was highly expressed, suggesting that MSCs could effectively promote the proliferation activity of EPCs. Echocardiography showed that after MSCs+EPCs treatment, the left ventricular ejection fraction, end-diastolic volume and cardiac volume of the rats were significantly improved, with statistically significant differences from the control group and the EPCs group (P<0.05), Meanwhile, the expression of VEGF in MSCs+EPCs group was also significantly increased (P<0.05). Mesenchymal stem cells can enhance the therapeutic effect of EPC cells in ischemic heart disease, which can promote myocardial angiogenesis, improve ventricular remodeling and cardiac function.

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Article: Longitudinal analysis of trends and reporting quality of statistical methods of observational studies published in high-impact urology journals: a protocol for systematic assessment
by  ShuangYang Dai, BeiBei li, JinGao Zhang, XiaoBin Zhou
Chronic Diseases Prevention Review 2019 3(12) 6-14; published online 18 July 2019
Abstract:  Observational studies play an important role in urology research. The quality of statistical reporting can directly affect the reliability of observational studies. According to previous studies, the quality of statistical reporting for published biomedical literature tends to be low. There are no studies on the evaluation of the quality of statistical reports on urological observational studies. The purpose of this study is to investigate the trends and evaluate the reporting quality of statistical methods of observational study published in high-impact urology journals, compare with general medical journals in order to further identify factors related to high quality statistical reporting. This study plans to search the top five journals of urology and top two general medical journals with the highest 5-year impact factor (based on ISI Web of Knowledge Journal Citation Reports) in Pubmed and EMBASE. All observational studies that meet the inclusion criteria will be included. Table “common statistical methods in medical studies” will be used to assess the frequency and trends of statistical methods for observational studies published in urology journals. The quality of statistical reporting for each article will be assessed according to Table “the assessment checklist of the reporting quality of statistical methods”. The table consists of 7 items (marked *) that must be reported and 39 items that are subject to selective reporting based on the statistical methods used in the article. The proportion of fully reported items will be calculated based on the type of statistical method used in each article. A logistic regression model will be established to identify factors associated with high-quality statistical reporting. This study will identify major deficiencies of statistical reports, which may provide a reference for authors, reviewers and editors of journals, and help to improve the statistical reporting quality of articles in urological journals.

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Article: Analysis on influencing factors for the cure of pulmonary tuberculosis patients in adolescents
by  Yuxia Sun, Jianwen Zhang, Chongxiu Sun, Mengru Jia, Yanyan Kang, Aiguo Ma
Chronic Diseases Prevention Review 2019 3(12) 15-19; published online 18 July  2019
Abstract:  To explore the influencing factors of adolescent tuberculosis patients, and to provide a basis for improving the cure rate of adolescent tuberculosis patients. The clinical data of adolescent tuberculosis patients aged 14-35 in the tuberculosis control centers of 7 counties in Linyi area of Shandong Province from 2009 to 2013 were collected. Logistic regression model was used to analyze the influencing factors of adolescent tuberculosis patients. A total of 706 adolescent pulmonary tuberculosis patients were included in this study, of whom 669 were cured and 37 were not cured. Multiple Logistic regression analysis showed that pulmonary cavity (OR=3.190, 95%CI=1.433-7.054), complications (OR=8.826, 95%CI=3.327-23.414), sputum test results at the end of second month (OR=0.112, 95%CI=0.033-0.386) and treatment classification (OR=13.737, 95%CI=4.682-40.304) could affect the cure of pulmonary tuberculosis in adolescents. We should raise attention for adolescent tuberculosis patients. During the treatment of tuberculosis, evaluate and actively intervene in adolescent tuberculosis patients with pulmonary cavity, comorbidities, positive sputum test at the end of second month and retreatment, so as to effectively improve the cure rate of adolescent tuberculosis patients and prevent the spread and prevalence of tuberculosis.

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Article: Prevalence and related risk factors of hypertension and normal-high blood pressure in patients with type 2 diabetes mellitus admitted to hospital: a cross sectional study
by  Xiuping Du, Shuhui Nie, Xiang Li, Xiangling Cai, Qiuzhen Wang
Chronic Diseases Prevention Review 2019 3(12) 20-27; published online 18 September  2019
Abstract:  With the increase of China's population and economic development, diabetes (DM) has become a serious public health problem, the complications of DM are also serious. Among them, patients with DM complicated with hypertension have to face the double risk of infection and cardiovascular disease. Not only the cost of medical care has increased, but also the quality of life has declined. The purpose of this study was to compare the differences in lifestyle and body characteristics between patients with DM complicated with hypertension, DM with normal-high blood pressure and patients with DM without normal-high blood pressure, and to provide clues for the inference of risk factors related to DM complicated with hypertension. A cross-sectional study was conducted T2DM patients hospitalized in a hospital from January 2017 to December 2017. One-way ANOVA and chi-square test were used to judge the difference between the two groups. A total of 79 patients with simple DM, 92 patients with normal-high blood pressure and 138 patients with DM complicated with hypertension were enrolled in our study. We found differences in age, neutrophil count, lymphocyte ratio, uric acid (UA), creatinine (CRE), total protein (TP), glycosylated hemoglobin (HbA1c), body mass index (BMI), course of disease and self-control between the two groups. We found that the age, body mass index (BMI) and inflammatory response in diabetic patients with hypertension were significantly higher than those in patients with simple DM and DM with normal-high blood pressure. However, the self-control of patients with hypertension is better than that other groups. Therefore, with the prolongation of illness time, patients need to control the occurrence of complications through self-control and weight loss.

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Article: Glucose fluctuation during insulin therapy in diabetic patients with nasal feeding analysis of influencing factors
by  Wane Zhao, Xiaohua Lu, Yan Zhou, Jiewei Huang, Chuangbiao Zhang, Yan Liu, Yu Liu, Xixi Luo
Chronic Diseases Prevention Review 2019 3(12) 28-33; published online 28 September  2019
Abstract: To explore the factors related to blood sugar fluctuation in diabetic patients with continuous nasal feeding during insulin pump therapy, and to provide clinical reference for the adjustment of insulin therapy. Forty diabetic patients with continuous nasal feeding treated with insulin from January 2016 to June 2019 in our hospital were selected. The blood sugar fluctuation was observed by 7-8 fingertip blood monitoring every day. The blood sugar fluctuation of patients with continuous nasal feeding was observed by analyzing the clinical data. In addition, the related factors affecting blood sugar fluctuation were summarized. Using LAGE and SDBG as the evaluation indexes of blood sugar fluctuation, logistic multiple regression analysis of single and multiple factors was carried out for the possible related factors affecting blood sugar fluctuation.The blood sugar levels of different sex, age, course of disease, glycosylated hemoglobin, body mass index (BMI) and other indicators of patients were compared, and no statistical difference was found in the blood sugar fluctuation levels of gender, age and BMI groups (p>0.05). With the increasing of diabetes course, the blood sugar fluctuation amplitude increased (P < 0.05). Carbohydrate content and total protein during the period also lead to blood sugar fluctuation (P < 0.05). Unconditional multivariate logistic regression analysis showed that duration (OR = 1.201, P = 0.016), carbohydrate (OR = 1.029, P = 0.016), total protein (OR = 1.134, P = 0.046) were the factors of blood sugar fluctuation in diabetic nasal feeding patients. For diabetic patients with continuous nasal feeding, the fluctuation of blood sugar is related to the course of disease, carbohydrate content, total protein and other factors. For those patients with risk factors, we should take relevant measures and pay attention to the fluctuation of blood sugar.

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