25. 10. 2019         

Chronic Diseases Prevention Review (Online ISSN: 2158-0820)


Current Issue

Vol.3 No.12


Article: Vivo study of MSCs on EPCs cells in ischemic heart disease
by  Jianming Wang, Gengbiao Chen, Youfeng Guo, Xiaohong Yin, Xiaodong Wang
Chronic Diseases Prevention Review 2019 3(12) 1-5; published online  18 July  2019
Abstract:  The treatment of ischemic heart disease mainly depends on the formation of blood vessels and the survival of cardiac myocytes. This paper will research the enhancement of EPC cells by mesenchymal stem cells in the treatment of ischemic heart disease. Mouse bone marrow was collected, MSCs cells were isolated and cultured. Flow cytometry was used to test the expression of CD34 and CD44. Then MSCs cells and endothelial progenitor cells co-culture 48 h, and 24 Wistar rats were randomly divided into EPCs group, EPCs MSCs + group and the control group. MSCs 2×106, EPCs 2×106, EPCs 2×106, and PBS buffer were injected into five different regions around ischemic myocardium. The changes in cardiac function were assessed by echocardiography after 4 weeks. MSCs 2×106, EPCs 2×106, EPCs 2×106, and PBS buffer were injected into five different regions around ischemic myocardium. The changes in cardiac function were assessed by echocardiography after 4 weeks. The rats were sacrificed, and the expression of VEGF was detected by immunohistochemistry and immunofluorescence. The surface markers CD34 and CD44 of MSCs and EPCs were differentially expressed. We successfully isolated MSCs and EPCs cells. Meanwhile, the proliferation index Ki67 was highly expressed, suggesting that MSCs could effectively promote the proliferation activity of EPCs. Echocardiography showed that after MSCs+EPCs treatment, the left ventricular ejection fraction, end-diastolic volume and cardiac volume of the rats were significantly improved, with statistically significant differences from the control group and the EPCs group (P<0.05), Meanwhile, the expression of VEGF in MSCs+EPCs group was also significantly increased (P<0.05). Mesenchymal stem cells can enhance the therapeutic effect of EPC cells in ischemic heart disease, which can promote myocardial angiogenesis, improve ventricular remodeling and cardiac function.

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Article: Longitudinal analysis of trends and reporting quality of statistical methods of observational studies published in high-impact urology journals: a protocol for systematic assessment
by  ShuangYang Dai, BeiBei li, JinGao Zhang, XiaoBin Zhou
Chronic Diseases Prevention Review 2019 3(12) 6-14; published online 18 July  2019
Abstract:  Observational studies play an important role in urology research. The quality of statistical reporting can directly affect the reliability of observational studies. According to previous studies, the quality of statistical reporting for published biomedical literature tends to be low. There are no studies on the evaluation of the quality of statistical reports on urological observational studies. The purpose of this study is to investigate the trends and evaluate the reporting quality of statistical methods of observational study published in high-impact urology journals, compare with general medical journals in order to further identify factors related to high quality statistical reporting. This study plans to search the top five journals of urology and top two general medical journals with the highest 5-year impact factor (based on ISI Web of Knowledge Journal Citation Reports) in Pubmed and EMBASE. All observational studies that meet the inclusion criteria will be included. Table “common statistical methods in medical studies” will be used to assess the frequency and trends of statistical methods for observational studies published in urology journals. The quality of statistical reporting for each article will be assessed according to Table “the assessment checklist of the reporting quality of statistical methods”. The table consists of 7 items (marked *) that must be reported and 39 items that are subject to selective reporting based on the statistical methods used in the article. The proportion of fully reported items will be calculated based on the type of statistical method used in each article. A logistic regression model will be established to identify factors associated with high-quality statistical reporting. This study will identify major deficiencies of statistical reports, which may provide a reference for authors, reviewers and editors of journals, and help to improve the statistical reporting quality of articles in urological journals.

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Article: Analysis on influencing factors for the cure of pulmonary tuberculosis patients in adolescents
by  Yuxia Sun, Jianwen Zhang, Chongxiu Sun, Mengru Jia, Yanyan Kang, Aiguo Ma
Chronic Diseases Prevention Review 2019 3(12) 15-19; published online 18 July  2019
Abstract:  To explore the influencing factors of adolescent tuberculosis patients, and to provide a basis for improving the cure rate of adolescent tuberculosis patients. The clinical data of adolescent tuberculosis patients aged 14-35 in the tuberculosis control centers of 7 counties in Linyi area of Shandong Province from 2009 to 2013 were collected. Logistic regression model was used to analyze the influencing factors of adolescent tuberculosis patients. A total of 706 adolescent pulmonary tuberculosis patients were included in this study, of whom 669 were cured and 37 were not cured. Multiple Logistic regression analysis showed that pulmonary cavity (OR=3.190, 95%CI=1.433-7.054), complications (OR=8.826, 95%CI=3.327-23.414), sputum test results at the end of second month (OR=0.112, 95%CI=0.033-0.386) and treatment classification (OR=13.737, 95%CI=4.682-40.304) could affect the cure of pulmonary tuberculosis in adolescents. We should raise attention for adolescent tuberculosis patients. During the treatment of tuberculosis, evaluate and actively intervene in adolescent tuberculosis patients with pulmonary cavity, comorbidities, positive sputum test at the end of second month and retreatment, so as to effectively improve the cure rate of adolescent tuberculosis patients and prevent the spread and prevalence of tuberculosis.

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