Cancer Cell Research (Online ISSN: 2161-2609)
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Current Issue
Vol.2 No.6
Article: Chronopharmacology of Antitumor Effect of Gefitinib in Tumor-Bearing Nude and Its Mechanism1
by Liang Liu, Mingchun Li
Cancer Cell Research 2015 2(6) 125-128; published online 19 January 2015
Abstract: The aim of this study was to investigate the
influence of different time points on the efficacy, and
investigate its underlying mechanism. We established
the non-small cell lung cancer model by BALB/c Nude, and
then nude were randomly divided into gefitinib groupsand
model group. Group gefitinib were given 1mg/kg gefitinib
intragastrically at 4:00, 8:00, 12:00, 16:00, 20:00, 24:00,
the model group received the same volume of 1%Tween-80
solution. The change of tumor volume in 20 days and tumor
weight on the 21th day of mice were measured. The mRNA
expressions of EGFR, AKT, ERK1/2 and mTOR in tumor tissue
were detected. Compared with the model group, the
tumor of group gefitinib mice grow slower(P <0.05), in which
the group 8:00 is slowest, group 20:00 is fastest (P <0.05);
the tumor mass of groups gefitinib were lower than the model
group(P <0.05), the inhibition rate of group 8:00 was the
highest, 20:00 group was the lowest; the lowest mRNA
expression of EGFR, AKT, ERK1/2 and mTOR was group 8:00
group 20:00 was the highest. Gefitinib can
inhibit the tumor of bearing mice, and have time rhythm, the
best time of inhibitory effect is 8: 00, and the worst time
of inhibitory effect is 20:00.
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Article: CT and MRI Diagnosis of Osteolytic Metastases of the Vertebral Column
by Xiaonan Cai, Jihua Liu
Cancer Cell Research 2015 2(6) 129-133; published online 20 January 2015
Abstract:
To analyze the CT and MRI features of spinal osteolytic
metastases to investigate the diag nostic value of CT and
MRI images of this disease. CT and MRI imaging features of
50 patients with spinal osteolytic metastases confirmed
pathologically were analyzed retrospectively. CT imaging
features: 72 vertebrae were involved in 50 patients, 32
vertebrae showed a rounded soft tissue low density area, 40
vertebrae showed an irregular sheet of soft tissue low
density area. Fifty-three of these 72 vertebrae were
surrounded with sclerotic margins which were thinner than 2
mm. In 39 vertebrae the cortex was destroyed, while in 11
vertebrae the cortex had expanded into an incomplete
crustiform structure. Eight vertebrae were fragmented and
appeared as mottling, with imaging showing that the outline
of the vertebrae had disappeared, with burst-like bone
fragments scattered around, and the vertebrae exhibiting
mixed density. Twelve vertebrae were associated with
pathologic compression fractures. There were 35 paraspinal
soft tissue masses. MRI imaging features: of the 72
osteolytic metastatic vertebrae shown in CT, 62 vertebral
lesions showed low signal in T1WI, while T2WI showed a
heterogeneous high signal, and T2WI fat-suppressed images
also showed a high signal, the 53 vertebrae surrounded by
sclerotic margins showed corresponding low signal rings in
T1WI. Osteolytic spinal metastases each have their own
characteristics in CT and MRI imaging. CT has the ability to
make accurate judgments on sclerotic changes in the
vertebrae, while MRI is more sensitive to paraspinal soft
tissue swelling, soft tissue masses and bone marrow edema.
It becomes a specific performance that there ubiquity
sclerosis around the lesions. The combination of both CT and
MRI can provide accurate and reliable clinical information,
and improve the rate of accurate diagnosis.
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Article: Three bioactive components determination by TLC and HPLC for Compound Shanzha Granules
by Yanwei Fu, Songgang Ji, Mingchun Li, Yan Ma, Yanqin Cheng
Cancer Cell Research 2015 2(6) 134-139; published online 22 January 2015
Abstract:
To establish the quality standard of compound Shanzha
Granules. The chief components of the preparation, Crataegi
Fructus and Cassiae Semen were identified by TLC. The
contents of Autrantio-obtusin and Chrysophanol in Cassiae
Semen were determined by HPLC. The separation was performed
on Agilent SB-C18 (4.6×250mm, 5μm) with acetonitrile-0.1%
phosphoric acid as mobile phase for gradient elution. The
relevant spots on TLC plates were clear by the blank
reference. The contents showed good linear, it is in the
range of 0.0357～0.3570µg (r=0.9999) for Autrantio-obtusin
and 0.0520～0.5200µg (r=0.9999) for Chrysophanol. The average
recovery rate was 99.64% for Autrantio-obtusin and 100.18%
for Chrysophanol. The established qualitative and
quantitative methods are simple, accurate and reproducible,
which can be used for the quality control of compound
Shanzha Granules.
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Article: 3.0TMRI Diffusion Weighted Imaging in Assessing the Efficacy of Cervical Cancer Chemo-radiotherapy
by Jia Yang, Weihua Feng, Hui Hua, Xianglong Shi, Qinglian Ji, Jingjing Chen
Cancer Cell Research 2015 2(6) 140-143; published online 26 January 2015
Abstract:
To analyze DWI magnetic resonance imaging both anterior and
posterior to cervical cancer chemotherapy and to explore the
application of ADC and EADC values in predicting and
monitoring the efficacy of cervical cancer chemotherapy.
Perform conventional MR scanning and DWI on 52 cases of
cervical cancer patients prior to chemotherapy and fifteen
days, one month, and two months into chemotherapy. At
different check points, measure ADC and EADC values and the
maximum diameter of the tumor. Analyze differences in ADC
and EADC values before and after chemotherapy. ADC changes
take place earlier than morphological changes in tumor
volume. ADC values are significantly higher than those
before treatment while EADC values get lower. 2 months into
the treatment, the reduction rate of the maximum tumor
diameter is negatively correlated to ADC values before the
treatment (r = -0.658, P <0.05). 15 days into the treatment,
the mean ADC values increase (t = 11.119, p <0.05). EADC
values decrease (t = -9.916, p <0.05). And the maximum tumor
diameter shows no significant change from that before the
treatment (t = -1.797, p> 0.05). ADC and EADC values before
cervical cancer chemotherapy may help predict the efficacy
of cancer treatment. ADC and EADC values during the
treatment may contribute to early detection and dynamic
observation of therapeutic effect.
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