Friday, 25. 5. 2020

Cancer Cell Research (Online ISSN: 2161-2609)

Current Issue

Vol.7  No.27

Article: Second generation sequencing was performed to detect the gene mutation of PIk3CA in gynecological tumors and its relationship with the prognosis of patients
by Yuanli Guo, Junfeng Liu,Chen Shan
Cancer Cell Research 2020 7(27) 714-719; published online  15 July 2020
Abstract: To investigate the gene mutation of PIk3CA in gynecological tumors and analyze the relationship between PIk3CA and median survival time. Our study included gynecologic tumor patients 110 cases ((40 cases of cervical cancer, 40 cases of endometrial carcinoma, 30 cases of ovarian cancer) from January 2011 1 to August 2019, the First Affiliated Hospital of Sun Yat-sen University and Sun Yat-sen University Affiliated Hospital of Department of Gynaecology. Paraffin tumor samples from patients with postoperative, cut out the white piece, extract DNA, using the second generation sequencing of high-throughput sequencing platform, access to genetic testing results, the Kaplan-Meier survival curve analysis state of PIk3CA gene and the relationship between gynecological malignant tumor patients' overall survival time. In 110 patients with gynecological tumor, 61 cases (55.45%) patients had PIk3CA gene mutations, consensus don't 9745 variants, including 7215 single nucleotide mutation and 2530 missing or insertion mutation, type of PIk3CA mutations in patients with cervical cancer and ovarian cancer gene amplification, highly PIk3CA mutations in endometrial carcinoma was 36.67% (11/30), cervical cancer was 57.5% (23/40), ovarian cancer was 67.5% (27/40), compared with tissue adjacent to carcinoma (P < 0.05). The follow-up period was June 1, 2019, and a total of 98 patients were followed up. In cervical cancer and ovarian cancer, the poor prognosis of patients with PIk3CA gene mutation was significantly reduced in the non-mutation group (P=0.001), but no correlation was found between PIk3CA gene mutation and the survival time of patients in endometrial cancer. PIk3CA gene is highly extended in gynecological tumors, especially in ovarian cancer and cervical cancer, and is closely related to the poor prognosis of patients, which is great value for guiding clinical prognosis and guiding precise treatment.

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