Cancer Cell Research (Online ISSN: 2161-2609)

by Yuanchuan He, Zenghui Lu, Ping Shi, Zongjie Zhao, Shijiang Chen

Cancer Cell Research 2015 2(8) 185-191; published online 6 October 2015

Abstract: Antrodia camphorata is a rare and precious parasitic fungus. A great deal ergostanne and lanstane triterpenoid were isolated and elucidated. This review discussed relationship of chemical structure and bioactivities, anticancer effect and possible mechanism of triterpenoids compounds, and related pharmacological effects, such as anti-inflammatory, immunomodulatory, hepatoprotective and antioxidant activities with various pathways in vitro and animal experiments.

by  Xinxin He, Ximing Shen, Dongxia Wu, Qing Sun

Cancer Cell Research 2015 2(8) 192-196; published online 25 November 2015

Abstract: IGF-IR Antisense Oligodeoxynucleotide (ASODN) was synthesized and added to breast cancer MDA-MB-231 cells to see its influence on cell proliferation and angiogenesis. After the transfection of IGF-IR ASODN on the MDA-MB-231 cells, immunocytochemical method, western blot and RT-PCR methods were used to test the IGF-IR, VEGF and PCNA level. Cell proliferation was analyzed by MTT method. The results showed that after transfection 2-8 µM IGF-IR ASODN can inhibit expression of IGF-IR and cell proliferation. The inhibition effect begun at 24 hours and lasted to 96 hours.The inhibition effect was related to the concentration. IGF-IR ASODN could also inhibit protein level of VEGF and PCNA. Promoting autocrine and paracrine, IGF-IR is important to breast cancer cells growth and angiogenesis ability.

by  Xiuhua Yang, Hongquan Chen

Cancer Cell Research 2015 2(8) 197-200; published online 25 November 2015

Abstract:  To investigate the protective effect of Paeoniflorin on UVB-induced apoptosis of HaCat cells. HaCat cells were divided into eight groups, including group A: control group; group B: UVB irradiation group; group C: 0.25mg/L Paeoniflorin experimental group; group D: 0.5 mg/L Paeoniflorin experimental group; group E: 2.5 mg/L paeoniflorin experimental group; group F: 5 mg /L paeoniflorin experimental group; group G: 10 mg/L paeoniflorin experimental group and group H: 12.5 mg/L paeoniflorin experimental group. In addition to the group A, the other groups were irradiated by UVB. The apoptosis rate was analyzed by the flow cytometry. RT-PCR was used to detect the expression levels of P21mRNA. Compared with the group B(4.840±0.236), the apoptosis rate of group C~group F(0.423±0.179, 1.127±0.235, 2.570±0.239, 3.137±0.347) was significantly reduced. The differences were statistically significant (P <0.05); while compared with the group B, the apoptosis rates of the group G and the group H were not statistically significant (P> 0.05); Compared with the group B (1.040±0.007), the expression of P21mRNA of group C~group H (0.440±0.015, 0.551±0.013, 0.848±0.027, 0.850±0.052, 0.923±0.035, 0.936±0.041) was significantly decreased, and the differences were statistically significant (P <0.05). From this experiment, it can be speculated that paeoniflorin have a protective effect on UVB-induced apoptosis of HaCat cells in a certain concentration (0.25 mg/L ~ 5 mg/L), and it can reduce the expression of P21mRNA of Hacat cells.

by  Cuizhu Ge, Fucheng Song, Hua Gao, Honglin Zhai, Hongzong Si

Cancer Cell Research 2015 2(8) 201-209; published online 25 November 2015

Abstract:  To explore the structure activity correlation of quinazoline derivatives as EGFR-T790M inhibitors, three-dimensional quantitative structure-activity relationship (3D-QSAR) modeling were carried out. A satisfactory model with good predictive abilities have been developed by CoMFA, the cross validated Q2=0.651, non-cross-validated R2=0.988. Based on these results, we design novel compounds as potential inhibitors of EGFR-T790M with good predicted activities. The results of 3D-QSAR will guide the research and development of novel EGFR-T790M kinase inhibitors.