Cancer Cell Research (Online ISSN: 2161-2609)

by  Xu Su, Weifang Yang, Liguo Qi, Limei Lou, Lili Jiang, Bo Zhu, Huai Chen, Jian Yin, Qingping Lv, 

      Fuchuang Qin, Xuhong Ji, Kang Zheng, Wei Zhu, Song Zhang

Cancer Cell Research 2018 5(18) 435-438; published online  5 March 2018

Abstract: Primary intracranial myxoid chondrosarcoma (PIMCS) is extremely rare, with only seven patients previously have been reported. We present a case report of a 58-year-old woman admitted for numbness in her right face. However, there is no symptoms of increased intracranial pressure (ICP). Magnetic resonance imaging (MRI) revealed a well-enhanced large mass around her middle and posterior cranial fossa (Figure 1-4). A right pterional craniotomy was performed. Subtotal surgical resection was also performed, and pathology results confirmed an extraskeletal myxoid chondrosarcoma (Figure 5). Postoperative MRI showed some residual tumor, and the patient underwent radiotherapy. So far, the patient recovers well without discomfort complaints. This malignant tumor showed high rates of recurrence in previous reports. We here report anotheroccurrence of this highly malignant and rare tumor in a patient treated using subtotal surgical excision and adjuvant radiotherapy.

by  Zhanghai He, Baoxuan Li

Cancer Cell Research 2018 5(18) 439-441; published online  10 March 2018

Abstract: To study the effect and possible mechanism of endoplasmic reticulum stress for invasion and migration of the GIST cell. Using 10% fetal bovine serum to subculture GIST882 cell lines, using garment toxin to induce GIST882 for endoplasmic reticulum stress reaction, and then total protein were extracted and detected GRP78 and MMP-9 expressionsusing western blot. The healing method was usedto detect the cell of migration ability and Transwell method was used to detect the cell of GRP78 cells of invasionability. Compared with before induction, the expression levels of GRP78 protein after induced for 12h and 24h were significantly increased with time (P<0.05). Compared with the uninduced group, the cell migration and invasion ability in endoplasmic reticulum stress group were significantly enhanced, and the expression level of MMP protein was also significantly increased (P<0.05). Gastrointestinal stromal tumor has endoplasmic reticulum stress, and it may increase the expression of MMP–9 to promote cell invasion and migration.

by  Yanfang Liang, Longbin Cao, Bihua Lin, Can Chen, Jianbo Ruan, Maofu Wei, Keyuan Zhou, Jincheng Zeng 

Cancer Cell Research 2018 5(18) 442-445; published online  25 March 2018

Abstract: Extramammary paget's disease (EMPD) is a rare malignant neoplasm. Especially, EMPD associated with cancer is extremely rare. Here, we report a rare case of EMPD associated with sweat gland adenocarcinoma (SGA) located in the right groin of a 73-year-old Chinese man. The histology of the excised lesion revealed a focus of sweat glands like-Paget's cells and lower degree of differentiated carcinoma were observed on subcutaneous. The goblet cells of the skin stained strongly with alcian blue (AB) and periodic acid schiff (PAS). Laboratory tests revealed the man had an obviously rising CA125 level (46.58 U/ml), and tissues were immune-positive for CK, CK5/6, CK7, CEA, CK19, GCDFP-15, Keratin, P53, P63, Ki-67, ER, and negative for HMB45, PR, CK20, Melan A. The report concludes that immunohistochemistry is required to confirm the diagnosis and differentiation of EMPD combined with SGA from pagetoid SGA and EMPD.

by Ming Liu, Yuhe Duan, Renjie Chi, Fujiang Li, Hongting Lu 

Cancer Cell Research 2018 5(18) 446-451; published online  28 March 2018

Abstract: The purpose of this study is to investigate the effect of microRNA-483-3P (miR-483-3P) on proliferation, invasion and migration of neuroblastoma(NB) cells, and to validate the target genes. MiRNA microarray chips were analyzed. Negative control sequences of chemically synthesized miR-483-3P inhibitor and miR-483-3P inhibitor were transfected into SH-SY5Y cell line using cationic liposome LipofectamineTM 2000. Expression of miR-483-3P in each group was detected by RT-PCR technique. Proliferation of cancer cells in each group was detected by CCK-8 assay. Invasion and migration of cancer cells in vitro detected by Transwell chamber assay. Bioinformatics software was used to predict the target gene of miR-483-3P, and luciferase reporter assay was used to test the target gene. Western-blot was used to verify the target gene. MiRNA microarray chip results showed that miR-483-3P was up-regulated in NB. The results showed that miR-483-3P inhibitor of the expression of miR-483-3P in SH-SY5Y cell line decreased. After transfection with miR-483-3P inhibitor of the proliferation of NB cells were inhibited. Ability of invasion and migration of NB decreased significantly after transfected with miR-483- 3P inhibitor. We found RIOK3 was a candidate target gene in this study. RIOK3 is one of the target genes of miR-483-3P. miR-483-3P can down-regulate RIOK3 expression and significantly promote the proliferation and migration of NB cells in vitro.