Cancer Cell Research (Online ISSN: 2161-2609)

by  Dandan Zhang, Zhihong Yang, Zhantao Liu, Jia Liu, Qian Lin, Ranran Liu, 

     Qingxia Ma, Hongqin Liu, Yuecheng Yang, Guohui Jiang

Cancer Cell Research 2016 3(10) 229-233; published online 25 April 2016

Abstract: Present studies have been investigted the inhibiting effect of oleanolic acid on cisplatin-resistant non-small cell lung carcinoma (NSCLC) cell growth and epithelial-mesenchymal transition (EMT). The MTT assay was employed to determine the effect of oleanolic acid (OA) on the viability of A549/CDDP cell line. Assays related to cell migration and invasion was performed to detect the migratory and invasive characteristics of A549/CDDP after treated with OA. The expression of ERCC1 protein, P-gp protein, E-cadherin protein and Vimentin protein were determined by immunocytochemistry and western blotting. These results suggested that OA could significangtly inhibit drug resistance. OA can not only inhibit cell proliferation of A549/CDDP, but also inhibit its EMT phenotype. This research will broaden the clinical application fields of oleanolic acid in the treatment of cancer.

by  Xuri Sun, Hongfei Xiang, Bohua Chen, Xuexiao Ma

Cancer Cell Research 2016 3(10) 234-240; published online 25 April 2016

Abstract: To explore the influence to lumbar lordosis of different operation for lumbar disc herniation, as lumbar lordosis has a great effect on keeping the spine sagittal balance. 632 cases of lumbar disc herniation were treated in our hospital from December, 2010 to December, 2014, of which 331 male and 301 female, aged from18-83 with the average age of 50.12, was followed up for 1-36 months with a mean follow-up of 5.29 months. According to the operation methods, we divided into 2 groups: fixation and fusion group, of which 584 cases, including 307 male and 277 female, aged from 18-83 with the average age of 50.81, was followed up for 1-36 months with a mean follow-up of 5.86 months, and non-fusion (percutaneous transforaminal endoscopic discectomy or traditional fenestration and discectomy ) group, of which 48 cases, including 24 male and 24 female, aged from 21-77 with the average age of 41.67, was followed up for 1-24 months with a mean follow-up of 5.29 months. The preoperative and postoperative lumbar curvature of the two groups was measured by Cobb’s angle. The conclusion is the lumbar curvature in fixation and fusion group has increased after surgery, especially in female, more than 50 years old, at last follow-up more than 6 months, and at operation lever L4/5. The lumbar curvature of male patients in non-fusion group was increased more than which of female patients after surgery, and the lumbar curvature of non-fusion group was increased after surgery at operation lever L5/S1.

by  Qiang Liu, Yugong Feng, Xiaoqing Du

Cancer Cell Research 2016 3(10) 241-245; published online 25 April 2016

Abstract: Our aim is to study intracranial aneurysm (IA) patients with rs6465976 single nucleotide polymorphism in elastin of Qingdao, China population patients IA correlations. A case-control analysis method, randomly selected from March 2015 to February 2016 in the Affiliated Hospital of Qingdao University, patients were from the department of neurosurgery of no blood relationship of intracranial aneurysm patients 60 cases as the research object, also randomly selected 60 controls of healthy unrelated volunteers as control group. The genotypes ELN rs6465976 in 60 IA and 60 controls were examined by TaqMan allelic discrimination real-time polymerase chain reaction (PCR). In the end, the portion of the blood sample randomly selected for PCR amplification and DNA sequencing again to verify the correctness of the result of. In the case group rs6465976 site GG, AG and AA three genotypes frequencies were 30%, 43.3% and 26.7%, besides the control group were 36.7%, 46.7%, 16.6% and difference was not statistically significant (P > 0.05). The frequency distributions of allele G and A were 51.67% and 48.33%, respectively. While the control group was 60% and 40% respectively, and the difference was not statistically significant (P>0.05) Thus ELN rs6465976 polymorphism may not be the risk factors of intracranial aneurysm formation.

by  Yuning Chen

Cancer Cell Research 2016 3(10) 246-250; published online 25 April 2016

Abstract: Cervical cancer is one of the most common diseases in female, seriously threat women's health, easily lead to cancer or death. This study was to explore early cervical cancer by screening of health examination population and risk assessment, and then to reduce the incidence of cervical cancer. In our hospital, 2002, early stage cervical cancer cases by screening of health examination population were the research object. Cervical cancer cases, colposcopy testing and pathological biopsy cases were given questionnaires. All the data were analyzed using single factor and multiple factor analysis method. Among the health examination population, 202 cases were given colposcopy testing in our hospital. 1800 cases were accepted the colposcopy testing and pathological biopsy. Among them, 150 cervical cancer patients were found. The clinical features of the tumor were the lower stage, the histological higher differentiation. The pathology types were mainly squamous cell carcinoma and adenocarcinoma. One way-ANOVA results showed that the early sage cervical cancer risk factors included: HPV infection, age at menarche and sexual life, personal hygiene habits, smoking, age and education level, which had little effect on cervical cancer. Multivariate logistic regression analysis showed that independent risk factors of cervical cancer were HPV infection or not (OR=2.023), age at menarche and sexual life (OR=1.347) and personal health habits (OR=1.248). HPV infection or not, age at menarche and sexual life, personal hygiene habits are independent risk factor for early cervical cancer. Screening and risk assessment have benefit for the prevention and treatment of cervical cancer occurrence and development, which also can effectively reduce cervical cancer complications and mortality.