Friday, 22.1.2016    

Cancer Cell Research (Online ISSN: 2161-2609)

Current Issue

Vol.3  No.9

Article: Clinical study of FOLFIRI treatment for gastric cancer patients with metachronous ovarian metastasis
by  Xiaodan Wang, Ping Sun
Cancer Cell Research 2016 3(9) 210-213; published online 25 January 2016
Abstract: To observe the short-term effects and adverse reaction of FOLFIRI for gastric cancer patients with metachronous ovarian metastasis. 30 cases of patients using the Irinotecan for 180 mg/m2, intravenous drip, d1; Leucovorin (CF) 200 mg/m2, intravenous drip, d1~2; 5-FU 400 mg/m2 intravenous drip, d1~2 and 2400mg/m2 using an ambulatory pump continuous infusion for 44-48 hours, repeat every 2 weeks; The therapeutic effects were evaluated after 3 cycles. In the 30 cases, 0 cases were CR, 6 cases were PR, 8 cases were SD, 16 cases were PD. The overall response rate was 20%, disease control rate was 46.7%. The most common adverse reactions was leukopenia, diarrhea, nausea and vomiting. The short term efficiency of FOLFIRI is good, and its adverse reactions are tolerable and can be controlled.

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Article: Study effect and mechanism of andrographolide on breast cancer cell MCF-7 and HCT116
by Songyan Hu, Youfeng Guo
Cancer Cell Research 2016 3(9) 214-218; published online 25 January 2016

Abstract: The aim of this study was to investigate the inhibitory effect of andrographolide on breast cancer cell line MCF-7 and HCT116 proliferation and its mechanism. MCF-7 and HCT116 in breast cancer cell were collected in vitro, according to different concentration of andrographolide (AND) dealt were divided into four groups, 15mol/LAND was as low concentration group, 30 mol/L AND was as middle concentration group, 60 mol/L AND was as high concentration group, no AND dealt was as blank control group, cell proliferation was detected by MTS, morphological change of cells were observed by microscope, apoptosis related proteins Caspase-3, Caspase-9 and Bal-2 mRNA express were detected by RT-PCR. The result showed that the cell proliferation inhibition rate of AND dealt groups were significantly increased, and with different concentration and dealt time changed significantly, AND concentration was high, the dealt time was longer, cell proliferation inhibition rate were higher (P<0.05), the microscope showed, MCF-7 and HCT116 cell shrinkage, shedding, assume apoptosis. With AND concentration increasing, Caspase-3, Caspase-9mRNA express gradually increased, Bal-2 mRNA express gradually weakened, comparison between groups with significant difference (P<0.05). Andrographolide on breast cancer MCF-7 and HCT116 cell proliferation has obvious concentration and time dependent inhibition effect, its mechanism may be down-regulating Bal-2 or up-regulating Caspase-3, Caspase-9 protein express.

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Article: The Expression and Significance of Angiopoietin-2 and Glucose Regulated Protein78 in Colorectal Cancer
by  Weiyan Du, Chen Qin, Jing Xu, Xuezhen Ma
Cancer Cell Research 2016 3(9) 219-222; published online 25 January 2016
Abstract: Colorectal cancer (CRC) is a severe health problem all over the world. To detect expression of Angiopoietin-2 (Ang-2) and Glucose Regulated Protein78 (GRP78) in colorectal carcinoma tissues, and their correlation with clinical pathological features, we examine expression of Ang-2 and GRP78 by immunohistochemical staining of paraffin-embedded tumor specimens from 70 colorectal cancer patients who underwent curative surgery at Qingdao Central Hospital. Through this experiment, the correlation of Ang-2 and GRP78 expression with clinicopathological parameters of colorectal cancer was analyzed. Ang-2 and GRP78 expression were detected in 68.57% and 74.29% of colorectal carcinoma respectively (P<0.05). Expression of Ang-2 and GRP78 were highly related to differentiation degree, lymph node metastasis, and clinical stages (P<0.05), but no significant correlation to the patients’ age, gender or the site and size of the lump was detected (P>0.05). Ang-2 and GRP78 are over-expressed in colorectal cancer, representing a molecular marker for malignant degree and infiltration metastasis of colorectal cancer. They play an important role in the occurrence and progression of colorectal cancer, thus providing new targets for targeted therapy of colorectal cancer.

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Article: Preparation and evaluation of liver targeting nano device based on Graphene Oxide mediated Miriplatin delivery
by  Chao Ma, Miao Yu, Guowen Yan, Ying Zhang, Chen Wei, Jiao Liu, Lutao Jiang, Jialing Dai, 
      Yitong Li, Yong Sun
Cancer Cell Research 2016 3(9) 223-229; published online 25 January 2016
Abstract: To prepare and evaluate a novel liver targeting nano device which based on graphene oxide (GO) loading miriplatin (MP). Glycyrrhetinic acid (GA) was linked to polyethylene glycol (PEG) via an amide linkage forming GA-PEG; then GA-PEG and GO were coupled by an ester linkage to form a triblock copolymer GA-PEG-GO (GPG). MP was packaged in liposome which conjugated to GO by means of modifying 1-Pyrenebutyric acid (PBA) on dioleoyl phosphoethanolamine (DOPE). Free MP was separated from liposome by 0.22μm filter membrane and determined by HPLC. The average size of nano device was 125.6 nm and average potential was +35.7 mV. The encapsulation efficiency (EE) of MP was 87.62%.

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