Cancer Cell Research (Online ISSN: 2161-2609)

by  Gengbiao Chen, Wei Lu

Cancer Cell Research 2017 4(13) 307-310; published online 3 January 2017

Abstract: To study the biology of hepatocellular carcinoma are restrained through PI3K/AKt signaling pathways and targeted by tumor infiltrating macrophages.Methods: Mononuclear cells were isolated from healthy adult peripheral blood by density gradient centrifugation and induced with IL - 4 for selective activation of macrophagesin vitro.PI3K - siRNA and AKT – siRNA were transfected to hepatocellular carcinoma cell line HepG2 respectively by lipofectermine 3000, and then cultivate with selective activation of macrophages (M2) for 48 hour. PI3K and Akt mRNA expression level in liver cancer HepG2 cells were detected with real-time fluorescent quantitative PCR. Results: After transfection, PI3K, and Akt mRNA level were significantly decreased. In vitro microenvironment of liver cancer, M2 can significantly increase levels of PI3K, and Akt mRNA levels in HepG2 cell lines, and can significantly promote the HepG2 cell proliferation ability (P< 0.05) after co-culture with M2 for 24 hours. Conclusion: Tumor associated macrophages can promote liver cancer cell proliferation through PI3K/Akt signaling pathway. If changing the microenvironment of liver cancer or specific inhibition of PI3K/Akt signaling pathway can also control the malignant biological behavior of tumors.

by  Nianju Zheng, Meixiang Li, Aiqin Song, Liang Song, Lirong Sun

Cancer Cell Research 2017 4(13) 311-315; published online 7 January 2017

Abstract: To investigate the proliferation and cell-cycle changes in the human RAJI cells after CHFR gene silencing. Cells were transfected with CHFR silencing RNA (shRNA) lentiviral vectors. CHFR silencing was verified in these cells 72h post-transfection using real-time polymerase chain reaction and western blot. Cell proliferation was analyzed by a standard Cell Counting Kit-8 (CCK-8) assay, and the cell cycle changes were detected by flow cytometry. The mRNA expression of CHFR in the ShRNA group was significantly down-regulated compared with that in the control and normal groups (both P<0.01). The results of western blot showed that CHFR protein expression decreased significantly compared with their corresponding levels in blank control group. The CCK results suggesting that the decrease in CHFR expression promoted cell proliferation(P<0.05). And Flow cytometry results demonstrated that the cell apoptosis rate was decreased after CHFR silencing (P<0.05). The decrease in CHFR expression led to promoted cell proliferation and the cell apoptosis rate was decreased. And CHFR gene may play a negative role during the growth and proliferation.

by  Tao Tang, Chunhua Li, Jin Chen, Gang Yang, Qiong Liao, Zhaohui Wang

Cancer Cell Research 2017 4(13) 316-319; published online 27 January 2017

Abstract: Primary peripheral nerve sheath tumors (PNSTs) of the thyroid are uncommon, and malignant peripheral nerve sheath tumors (MPNSTs) are extremely rare in the literatures. To the best of our knowledge, only three cases of MPNSTs have been previously reported in this location. This paper presents one case in a 30-year-old male, who had no history of neurofibromatosis or schwannoma, presented with a mass in the left lobe and underwent total thyroidectomy. The histological examination and immunohistochemical staining confirmed the mass a malignant PNST. After surgical excision, postoperative adjuvant radiotherapy with 6600cGy was administered. Twenty three months later, the patient was diagnosed lung and pleura metastasis by thoracic cavity biopsy, and then received three cycles of salvage chemotherapy with NVB and DDP. The patient finally died of respiratory failure secondary to severe pulmonary infection 29 months after the initial diagnosis of MPNSTs. Since primary thyroid MPNSTs share similar biological behavior with anaplastic thyroid carcinomas, we suggest that positive surgical excision of the aggressive tumor is essential, and the radiotherapy and chemotherapy are also needed.

by  Kaiqiang Qin, Xiaona Chu,Yongjang Wang, Liangdong Cheng, Chunqiao Li, Yuanbo Li, Liang Ning, 

       Shikuan Li

Cancer Cell Research 2017 4(13) 320-3325; published online 27 January 2017

Abstract: To assess the relationship between intestinal obstruction and the prognosis of rectal cancer. Data of 151 rectal cancer patients were extracted between January 2010 and October 2015 to survival analyse. The Kaplan-Meier method is used to survival analyse. The log-rank test was executed to compare the survival rate between patients with intestinal obstruction and those without intestinal obstruction. Cox proportional hazards regression analysis was performed to estimate the crude hazard ratio of mortality from colorectal cancer. The χ² test was used to compare disordered qualitative variables. The Kruskal Wallis H test was used to compare ordered qualitative variables. The 3-5 years overall survival rate after treatment of patients with intestinal obstruction were shorter than patients without intestinal obstruction respectively. The 3-5 years disease-free survival rate after treatment of patients with intestinal obstruction was lower than patients without intestinal obstruction, respectively. The log-rank test suggested that there were significant difference between the two overall survival curves (P=0.013) and two disease-free survival curves (P=0.047). Univariate and Multivariate Cox proportional hazards regression analysis suggested that intestinal obstruction decreased overall survival rate (P<0.05) instead of disease-free survival rate (P>0.05). Intestinal obstruction is a factor of poor prognosis of rectal cancer.