Cancer Cell Research (Online ISSN: 2161-2609)

Cancer Cell Research 2021 8(29) 778-783; published online  29 November 2021

Abstract: Exosomes are vesicle-like bodies with a diameter of 30 - 100 nm. They mediate intercellular signal transmission by transmitting their proteins, microRNAs (miRNAs) and long non-coding RNA (lncRNAs) to recipient cells. Esophageal cancer is a common malignant tumor of digestive system with high incidence rate and mortality. It lacks typical symptoms at early stage and has no mature early diagnosis. Therefore, if exosomes can be used as markers for early diagnosis, prognosis and efficacy evaluation of esophageal cancer, it can provide a new strategy for early diagnosis of esophageal cancer patients and the development of effective anti-cancer drugs in the future. in this research, we study the effect of exosomes in regulating the growth and metastasis, epithelial-mesenchymal transition, angiogenesis, tumor microenvironment and chemoresistance of esophageal squamous cell carcinoma (ESCC), which lays a solid foundation for the comprehensive understanding of the application of exosomes on the diagnosis and treatment of ESCC.

Cancer Cell Research 2021 8(29) 784-788; published online   21 December 2021

Abstract: It has been reported that G-protein signaling 17 (RGS17) oncogene is involved in the occurrence and development of ovarian cancer (OC), but the regulatory mechanism of OC is still unclear. MicroRNA (miRNA) negatively regulates gene expression, and its maladjustment is related to tumorigenesis. We understand the role of miRNA in OC modulators induced by RGS17. To characterize the regulatory effect of miR-203 on RGS17, we used ovarian cancer cell lines SKOV3 and OVCAR3, and constructed miR-203 and RGS17 overexpression vectors. CCK8 kit was used to detect cell proliferation, while Transwell analysis was used to measure cell invasion and migration. RT-qPCR and Western blotting were used to analyze the expression of miR-203 and RGS17 in ovarian cancer. The interaction between miR-203 and RGS17 was detected by fluorescent enzyme reporter gene method. The expression of miR-203 was significantly decreased in tumor cells, while the expression of RGS17 mRNA was increased. Luciferase reporter gene assay showed that miR-203 down regulated RGS17 expression by directly integrating into RGS17 mRNA. Our results showed that miR-203 was down regulated during tumorigenesis and inhibited the proliferation and invasion of OC cells. MiR-203 inhibits the proliferation, invasion and migration of ovarian cancer cell lines SKOV3 and OVCAR3 by targeting RGS17. After RGS17 overexpression, the regulatory effect of miR-203 was inhibited.

Cancer Cell Research 2021 829) 789-794; published online   23 December 2021

Abstract: To explore and observe the effect of DC-CIK maintenance therapy combined with traditional Chinese medicine treatment on the improvement of quality of life in patients with advanced non-small cell lung cancer (NSCLC). The research period was from January 2017 to April 2018. 89 patients with advanced NSCLC who were diagnosed and treated in our hospital. They were divided into observation group 49 cases and control group 40 cases. Record the improvement of the quality of life. After treatment, the short-term total effective rates of the observation group and the control group were 71.4% and 42.5% respectivel. The rate of the observation group was significantly higher than that of the control group (P<0.05). CD8+, CD3+ and CD4+ in the observation group and the control group after treatment were significantly different from those before treatment. And there was a statistically significant difference (P<0.05). DC-CIK maintenance therapy combined with traditional Chinese medicine treatment of advanced NSCLC patients can improve the quality of life, improve the body's immune ability, reduce the occurrence of adverse reactions, thereby improving the therapeutic effect.